You may decide to call numbers discretly as to not draw attention to the round 2 tryouts.
Excusing candidates and asking the "final cut" candidates to reamain is a procedure used in many sporting tryouts. You can have a second look at the team you are trying to build.
You can conduct "rounds" of cuts. If the second tryout is on a different day, you may see your candidates quite differently. This could be a great tryout tool.
It is hard to let go of a candidate that has the skill you desire or has the ability to perform at a higher level than the rest. Yet, coaches that have been around a while get quite familiar with the uncommitted "star" that misses practices, won't work at conditioning, bickers with teammates - and on and on. At some point it isn't worth it to try and build a team around an uncommitted person. This is especially true once you have had the joy of working with a group of unselfish kids that exceeded all expectations simply because they worked hard and worked together.